The whole plate can be detect in a single run with ease and flexible. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. 00. A. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Molecular Devices Software. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. SpectraMax® Microplate Readers and SoftMax® Pro Software. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofWe would like to show you a description here but the site won’t allow us. The adapter is required for optimum performa nce with standard 96-well and 384-. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Next generation SpectraMax® absorbance microplate readers. 5 µL of each sample was pipetted into a 96-well. The adapter is required for optimum performa nce with standard 96-well and 384-. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Create custom fluorescence assays without having to purchase expensive filter sets. VIS absorbance detection. SoftMax® Pro 7. Fig 2: Enzymatic response of synthetic compounds. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. Step 3: Click “Settings” and a screen will appear (Figure 1). FAST AND ACCURATE METERTECH Microplate Reader $3,650. 0 . Open the top cover of the instrument. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. SpectraMax® Microplate Readers and SoftMax® Pro Software. Step 1: Turn on your microplate reader. Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. 7°C and the bottom to 36. ** Some compatible filter sets are available for purchase from Life. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 304 signal level saturation. 1. System setup and calibration are controlled from within SoftMax Pro Software. To update the driver. Published Dec 16, 2020 | Updated Dec 16, 2020. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 1. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. This can increase throughput and efficiency, thus saving time and space in the lab. How to import latest protocol revisions into SoftMax Pro Protocol Library; Protocols for the ABS2 Validation Plate. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. The Versamax Microplate Reader is built around a grating monochromator. We would like to show you a description here but the site won’t allow us. Read one sample or up to 384 in a. Our systems for high-content imaging (HCI) and high-content analysis (HCA) provide flexible scalability making it easy to evolve your system alongside your research. PBS was removed, and cell pellets were. or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. Data acquisition and analysis. StakMax Handling Stacker Base Unit. 50/50. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax® Microplate Readers and SoftMax® Pro Software. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Expanded wavelength coverageAre you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. 0 × 103 to 2. Fibroblasts vs. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well. Spectranet customer portal. +1 800-635-5577. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. W×D×H. EUROPE. Some familiarity with Windows should be sufficient to use it. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SpectraMax ABS Plus reader utilizes Molecular . NOT FOR USE IN DIAGNOSTIC PROCEDURES. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The Beacon Melamine Plate Kit is a competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of melamine in contaminated samples. together with a Molecular Devices plate. Microplate Readers. View SoftMax Pro GxP. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. 1700 Online: Sales Offices USA/Canada 800. In this unique new five-part weekly webinar series, running every Thursday throughout October 2020, we’ll introduce you to various aspects of microplate reader technology in an educational and entertaining way! Starting with some basic concepts,. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. If it looks fine, then proceed. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 0A Sunnyvale, CA Made in USAFor SpectraMax ABS Plus reader, r 2 = 0. Eight filter options provide simplified assay setup. 2. . 18 kg. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Fluorescence Microplate Reader Comparison Chart. integration time per well, depending on firmware version. 303 unable to cal dark current. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 1 sec. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Citations on Google Scholar. The plate was then re-read on the plate reader (Read B). To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Page 1 of 8 . S. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. Imaging System Giles Scientific Biomic V3 Automatic Plate. An end-to-end solution for high-content imaging and analysis. in steps of 0. Built-in Help function answers your most frequently asked questions. Uncontrolled Copy for Training and Reference Purposes Only . The procedures for these tests are outlined in the instrument user manual. 58% for the detection module and 2. Additional high-quality filters to. ¨Automated microplate readers reduce manual manipulation and ensure consistent operation. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution for . Turn the reader on and secure the USB cable. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. These robust readers have been placed in labs from. ; SoftMax® Pro 7. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. In the M2, M5 and Plus. M5. Uncontrolled Copy for Training and Reference Purposes Only . Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Dimensions: 128mm (L) x 86mm (W) x 10mm (H). ®ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. Recertification service. SpectraMax Microplate Readers. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Feb 22, 2023Fast and compact absorbance microplate readers for a wide range of assays without the use of filtersSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. Incubate at RT. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. In . 40266 Email: drugdiscoverytech@lifetech. Select the Driver tab. Clone Screening. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. com #THX1138 Introduction The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 9000-0756. 1. The reader measures both flat and round 96-well microplates and. 9. Explore our fully-integrated,. #S1205. What are the physical and environmental operating specifications for SpectraMax UV/Vis and Gemini plate readers? (With extra info on backup power UPS choices). 25 to 3. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. 0 . Absorbance Plate Reader Comparison Chart. Fill a clean plate with water. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. Fig 2: Enzymatic response of synthetic compounds. SoftMax Pro Software. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic. #Flex3) Methods. The sample is typically placed in a cuvette through which light is sent horizontally. 1 sec. Instrument settings can be saved as a protocol file. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. Detection cartridge modularity allows for easy addition of new read modes within minutes without. For more sample throughput, the system can be easily integrated into a full roboticThe SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. , target CV per well = 1. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. Check pages 8-42 to 8-46 of the user manual. The former have been the traditional domain of plate readers and great strides have been. Instrument settings can be saved as a protocol file. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. Feb 22, 2023FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Details. 4: Test tube cover. or 0. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. 312 gain calibration failed. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The instrument settings were identical to those used in Example 1 above. Experimental details and customization of the group table. Eight filter options provide simplified assay setup. FlexStation® 3 Multi-Mode Microplate Reader. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. Fluorescence Microplate Reader Comparison Chart. Fluorescence Microplate Reader Comparison Chart. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. This can increase throughput and efficiency, thus saving time and space in the lab. Shop now. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for. The Spectratest® Abs1 plate, which is used for validationof Molecular Devices monochromator based readers, cannot be used to validate the Emax/Vmax readers. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 1-10 sec. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. After reading the ELISA plate on both readers, each standard curve was nearly identical. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 190. 00. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 306 plate air cal. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Molecular Devices Software. 0 × 107) RLU 0. Superior optical system includes a xenon flash lamp and features. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 더 많은. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. One plate carrier adapter is provided with the instrument. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. Multi-mode Microplate Readers. White plates reflect the light, enabling even low levels of Luminescence to be detected. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 9. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. FlexStation® 3 Multi-Mode Microplate Reader. We would like to show you a description here but the site won’t allow us. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. Keywords: spectramax ABS microplate readers, spectramax ABS plus microplate readers, spectramax ABS readers, ABS plus microplate readers, ABS. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 5577 UK +44. 9. Tutorial on how to quickly and easily view your results on the SpectraMax iD3 Multi-Mode Microplate Reader. SpectraMax Readers have the most complete level of product validation and compliance. 2. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. SoftMax® Pro 7. With various on-board features and. 00. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. First, as mentioned before, the walkaway time significantly. One plate carrier adapter is provided with the instrument. To update the driver. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). A control well was set up to verify the accuracy of the standard curve. Plate reader. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Plate section without PathCheck applied. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. 2. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 314 low lamp level warning. e. Contact our support team with your questions regarding Molecular Devices products and applications. Select Start>Control panel>Device Manager. The new assay will complement Molecular Devices’ workflow solution for cell line development by offering a 10-minute titer assay that can be run on the company’s SpectraMax series of microplate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The reader measures both flat and round 96-well microplates and. Perkin Elmer Victor X2 Multimode Microplate Plate Reader 2030-0020 w/ PC. Molecular Devices' microplate readers. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µl. Published Dec 16, 2020 | Updated Dec 06, 2021. Ratios for each sample were calculated as Read B/Read A. Exploring misconceptions and understanding the technologies. SoftMax Pro’s 21 curve fit options, including 4-parameter logistic and 5-parameter logistic models, and the ability to assign multiple standard curves within the same plate make it easy to interpolate unknown samples or perform parallel line analysis (PLA) to determine relative potency. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. SpectraMax® Microplate Reader Suite of Products Knowledge Base. 9. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. AXON Patch-Clamp. The SpectraTest® ABS1 Absorbance Validation Plate tests optical performance using NIST-traceable standards. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SoftMax Pro 7. Luminescence reading is a secondary mode of the Gemini instruments. SoftMax® Pro 7. Purchase a new SpectraMax Multi-Mode Microplate Reader and. 400×320×350 mm. Plus 384. Cellular Imaging Systems. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. 2. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. Select the Driver tab. Find regional support. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent. Testing can be done in the user’s lab on their own schedule. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Other features and benefits include: Eliminate changing out filters. M2. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. One plate carrier adapter is provided with the instrument. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Step 2: Launch SoftMax Pro Software. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. #0200-6182). Turn on Plate Reader and wait for it to complete its start-up routine. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1-2 sec. Search. The plate reader optics are. Integrated to. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Absorbance Plate Reader Comparison Chart. Molecular Devices provides web-based tutorials (webinars) and training courses throughout the year. Extended linear dynamic range. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Cells were thawed and propagated according to the recommended method specified by the supplier. 25 to 3. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. 0 . Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Cell Cytotoxicity assay procedureMolecular Devices. #P7589 or P11496) • Black 96-well plate (Greiner Bio-One, cat. 5. Recombinant protein expression Nucleocapsid. 6 % of reading 4. the washer with a final aspirate height of 4. Molecular Devices also offers Digital IQ/OQ/PM. Tristar 3 Multimode Microplate Reader. Absorbance Plate Reader Comparison Chart. If the computer is connected to the Internet: Select Search Automatically for. 1-1. Turn on Plate Reader and wait for it to complete its start-up routine. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Select Update Driver. In . 1 up to 3600 sec. S. 1. FlexStation™ II Systems provide integrated fluid transfer capability from a source platePlate Reader SpectraMax iD5 multi-mode system Plate Washer AquaMax 4000 Microplate Washer Robot PreciseFlex 400 Sample Handler Plate Storage Plate Hotels – 40 microtiter plates Controlling Software Genera Control Software Incubation LiCONiC STX44 Automated CO 2 Incubator Liquid Handler Microlab STAR Automated Liquid Handling The. 5 hours. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Figure 10: The Plate section Template Editor, showing the series set up. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535. The SpectraTest® ABS1 Absorbance Validation Plate**4 °C above ambient to 45 °C. For more sample throughput, both readers can be easily. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Molecular Devices microplate readers. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 211, and 820 and EudraLex Volume 4 and Annex 11 and 15, and how they apply to the implementation of Molecular Devices microplate readers and SoftMax Pro. performs within its specifications. 425 or. 8 foot) 4400-0002 Power cord, 1 meter (3. Vmax® plate reader specifications. Label-free detection. 997 (n = 3). FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. #X7060. HTRF-compatible SpectraMax Multi-Mode Readers. The primary mode is fluorescence. One plate carrier adapter is provided with the instrument. These systems are hybrids that allow you to choose between. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. View SoftMax Pro GxP. Molecular Devices’ AquaMax ® 4000 Microplate Washer aspirates. Read times. A kinetic plate read was initiated at time zero. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The lower limit of detection (LLD) was defined as the level of. SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models. 0) OD (0.